Philippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA

This volume is an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. During the past 17 years many new technologies or methodologies have appeared for the study of mouse development, and this volume comprehensively covers these, including new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.

Section I. General Resources1. A survey of internet resources for mouse developmentThomas L. SaundersSection II. Handling Mouse Lines2. Transport of mouse lines by shipment of live embryosKevin A. Kelley3. Strategies and considerations for distributing and recovering mouse linesYubin Du, Wen Xie, and Chengyu Liu4. Archiving and distributing mouse lines by sperm cryopreservation, IVF, and embryo transferHideko Takahashi and Chengyu LiuSection III. Gametes and Embryos5. Isolation and manipulation of mouse gametes and embryosEveline S. Litscher and Paul M. Wassarman6. Cryopreservation of mouse gametes and embryosCarlisle P. Landel7. Ovarian follicle culture systems for mammalsDavid F. Albertini and Gokhan Akkoyunlu8. Production of mouse chimaeras by aggregating pluripotent stem cells with embryosAndras Nagy, Kristina Nagy, and Marina Gertsenstein9. Production of cloned mice from somatic cells, ES cells, and frozen bodiesSayaka Wakayama, Eiji Mizutani, and Teruhiko Wakayama10. Nuclear transfer in mouse oocytes and embryosZhiming Han, Yong Cheng, Cheng-Guang Liang, and Keith E. Latham11. Culture of whole mouse embryos at early post-implantation to organogenesis stages: developmental staging and methodsJaime A. Rivera-Perez, Vanessa Jones, and Patrick P. L. Tam12. In utero and ex utero surgery on rodent embryosValerie Ngo-Muller and Ken MueokaSection IV. Fertilization13. Enhancement of IVF in the mouse by zona-drillingKevin A. Kelley14. ICSI in the mousePaula Stein and Richard M. SchultzSection V. ES and iPS Cells15. A simple procedure for the efficient derivation of mouse ES cellsEsther Wong, Kenneth Ban, Rafidah Mutalif, Nancy A. Jenkins, Neal G. Copeland, and Colin L. Stewart16. Producing fully ES cell-derived mice from 8-cell stage embryo injectionsThomas M. DeChiara, William T. Poueymirou, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, and David M. Valenzuela17. The loss-of-allele assay for ES cell screening and mouse genotypingDavid Frendewey, Rostislav Chernomorsky, Lakeisha Esau, Jinsop Om, Yingzi Xue, Andrew J. Murphy, George D. Yancopoulos, and David M. Valenzuela18. Induced pluripotent stem (iPS) cells Holm Zaehres, Jeong Beom Kim, and Hans R. SchölerSection VI. Imaging Mouse Development19. Imaging mouse embryonic developmentRyan S. Udan and Mary E. Dickinson20. Imaging mouse development with confocal time-lapse microscopySonja Nowotschin, Anna Ferrer-Vaquer, and Anna-Katerina Hadjantonakis21. Ultrasound and magnetic resonance microimaging of mouse developmentBrian J. Nieman and Daniel H. TurnbullSection VII. Hematopoiesis22. Use of transgenic fluorescent reporter mouse lines to monitor hematopoietic and erythroid development during embryogenesis Stuart T. Fraser, Joan Isern, and Margaret H. Baron23. Identification and in vivo analysis of murine hematopoietic stem cellsSerine Avagyan, Yacine M. Amrani, and Hans-Willem Snoeck

Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development.