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Autor: Roberta H. Smith
ISBN-13: 9780124159204
Einband: Taschenbuch
Seiten: 188
Gewicht: 340 g
Format: 227x154x15 mm
Sprache: Englisch

Plant Tissue Culture

Academic Press
Techniques and Experiments
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1. History of Plant Cell Culture

Trevor A. Thorpe

2. Setup of a Tissue Culture Laboratory

3. Media Components and Preparation

4. Explant Preparation

5. Contamination

6. Callus Induction

7. Regeneration and Morphogenesis

8. Woody Shrubs and Trees

Brent H. McCown

9. Haploid Plants from Anther Culture

10. Embryo Rescue

11. Meristem Culture for Virus-Free Plants

12. In Vitro Propagation for Commercial Production of Ornamentals

13. Protoplast Isolation and Fusion

Jungeun Kim Park, Sunghun Park, & James E. Craven

14. Agrobacterium-Mediated Transformation of Plants

Jungeun Kim Park, Sunghun Park, Qingyu Wu, & Stuart Sprague

Appendix I: Useful Measurements

Appendix II: Review of Solution Preparation

Appendix III: List of Suppliers

Appendix IV: Common Plant Tissue Culture Terms
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.
The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.

Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems
Includes detailed drawings that complement both introductions and experiments
Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology
Fully updated literature

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Autor: Roberta H. Smith
ISBN-13 :: 9780124159204
ISBN: 0124159206
Erscheinungsjahr: 13.10.2012
Verlag: Elsevier LTD, Oxford
Gewicht: 340g
Seiten: 188
Sprache: Englisch
Auflage 12003, 3. Auflage
Sonstiges: Taschenbuch, 227x154x15 mm