This timely reference discusses mass spectrometry in drug metabolism and pharmacokinetic studies. With contributions by professionals from the pharmaceutical industry, this book begins with a review of current mass spectrometry techniques and applications, followed by discussions of various methods for using MS in drug metabolism studies and pharmacokinetics. Highlighting the critical importance of ADME studies for understanding how a drug is absorbed, distributed, metabolized, and excreted by the body, the book s focuses on the use of LC/MS and MALDI-MS. This is a valuable reference for scientists in the pharmaceutical industry, medicine, academia, and even those working in homeland defense.

This timely reference discusses mass spectrometry in drug metabolism and pharmacokinetic studies. With contributions by professionals from the pharmaceutical industry, this book begins with a review of current mass spectrometry techniques and applications, followed by discussions of various methods for using MS in drug metabolism studies and pharmacokinetics. Highlighting the critical importance of ADME studies for understanding how a drug is absorbed, distributed, metabolized, and excreted by the body, the book s focuses on the use of LC/MS and MALDI-MS. This is a valuable reference for scientists in the pharmaceutical industry, medicine, academia, and even those working in homeland defense.

Preface viiAbout the Editor ixContributors xi1. Evolving Role of Mass Spectrometry in Drug Discovery andDevelopment 1Dil M. Ramanathan and Richard M. LeLacheur2. Quantitative Bioanalysis in Drug Discovery and Development:Principles and Applications 87Ayman El-Kattan, Chris Holliman, and Lucinda H. Cohen3. Quadrupole, Triple-Quadrupole, and Hybrid Linear Ion TrapMass Spectrometers for Metabolite Analysis 123Elliott B. Jones4. Applications of Quadrupole Time-of-Flight Mass Spectrometryin Reactive Metabolite Screening 159Jose M. Castro-Perez5. Changing Role of FTMS in Drug Metabolism 191Petia A. Shipkova, Jonathan L. Josephs, and Mark Sanders6. High-Resolution LC-MS-Based Mass Defect FilterApproach: Basic Concept and Application in Metabolite Detection223Haiying Zhang, Donglu Zhang, Mingshe Zhu, and Kenneth L.Ray7. Applications of High-Sensitivity Mass Spectrometry andRadioactivity Detection Techniques in Drug Metabolism Studies253Wing W. Lam, Cho-Ming Loi, Angus Nedderman, and DonWalker8. Online Electrochemical-LC-MS Techniques forProfiling and Characterizing Metabolites and Degradants 275Paul H. Gamache, David F. Meyer, Michael C. Granger, and Ian N.Acworth9. LC-MS Methods with Hydrogen/Deuterium Exchange forIdentification of Hydroxylamine, N-Oxide, and Hydroxylated Analogsof Desloratadine 295Natalia A. Penner, Joanna Zgoda-Pols, Ragu Ramanathan, Swapan K.Chowdhury, and Kevin B. Alton10. Turbulent-Flow LC-MS: Applications for AcceleratingPharmacokinetic Profiling and Metabolite Identification 311Joseph L. Herman and Joseph M. Di Bussolo11. Desorption Ionization Techniques for Quantitative Analysisof Drug Molecules 341Jason S. Gobey, John Janiszewski, and Mark J. Cole12. MALDI Imaging Mass Spectrometry for Direct Tissue Analysisof Pharmaceuticals 359Yunsheng Hsieh and Walter A. KorfmacherIndex 383