This book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with their use for drug delivery.

This book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with their use for drug delivery.

Foreword Current state of stem cell field: Overview (Mahendra S.Rao).Chapter 1: Derivation methods for human embryonic stem cells:Past, present & future Necati Findikli.Mohan Vemuri.Chapter 2: Isolation of human ESCs from various stages of thehuman embryo (Yuri Verlinsky, N. Strelchenko, V. Kukharenko, A.Shkumatov, S. Rechitsky, O. Verlinsky, and A. Kuliev).Chapter 3: Derivation of stem cells from epiblasts (MichalAmit).Chapter 4: Derivation of Embryonic Stem Cells fromParthenogenetic Eggs (Jose Cibelli).Chapter 5: Reprogramming Developmental Potential (Costas A.Lyssiotis Cradley D. Charette, and Luke L. Lairson).Chapter 6: Adult stem cells and their role in endogenous tissuerepair (N. Sachewsky and Cindi Morshead).Chapter 7: Greater differentiation potential of adult stem cells(Carlos Clavel and Catherine Verfaillie).Chapter 8: Cancer stem cells (Scott Dylla, In-Kyung Park andAustin L. Gurney).Chapter 9: Large scale production of adult stem cells forclinical use (Kristin Goltry, Brian Hampson, Naia Venturi andRonnda Bartel).Chapter 10: Genetic and epigenetic features of stem cells(Jonathan Auerbach and Richard Josephson).Chapter 11: Directed differentiation of embryonic stem cells(Marjorie Pick).Chapter 12: Identification of signaling pathways involved duringdifferentiation Takumi Miura.Chapter 13 Media and extracellular matrix requirements for largescale ESC growth (Allan J. Robins and Tom Schultz).Chapter 14: Automated method for culturing ES cells (S.Terstegge and Oliver Brustle).Chapter 15: Quantitative 2D Imaging of Human Embryonic StemCells (Steven K.W. Oh, Allen K. Chen, Andre B.H. Choo and IvanReading).Chapter 16: Nanobiotechonology for stem cell culture andMaintenance (Minseok S. Kim, Wonhye Lee and Je-Kyun Park).Chapter 17: Engineering Microenvironments to Control Stem CellFunctions (Anielle An-Chi Tsou and Song Li).Chapter 18: Improved lentiviral gene delivery tools for stemcells (Sanjay Vasu, Jian-Ping Yang and Wieslaw Kudlicki).Chapter 19: Sleeping Beauty-mediated Transposition in Stem Cells(Andrew Wilbur, Jakub Tolar, Bruce R Blazar, Catherine MVerfaillie, Uma Lakshmipathy, Dan S Kaufman and Scott McIvor).Chapter 20: PhiC31 Integrase for Modification of Stem Cells (W.Edward Jung and Michelle Calos).Chapter 21: Cell Engineering using Integrase and Recombinasesystems (Takefumi Sone, Fumiko Nishi, Kazuhide Yahata, YukariSasaki, Hiroe Kishine, Taichi Andoh, Ken Inoue, BhaskarThyagarajan, Jonathan D. Chesnut and Fumio Imamoto).Chapter 22: hESC derived cardiomyocytes for cell therapy anddrug discovery (William Sun and Robert Zweigerdt).Chapter 23: hESC in Drug discovery (Catharina Ellerstrom, PetterBjorquist, Peter Sartipy, Johan Hyllner and Raimund Strehl).Chapter 24: Characterization and Culturing of Adipose-DerivedPrecursor Cells (Dietmar Hutmacher, Joanna Olkowska-TruchanowiczDavid Leong, Johannes Reichert and Thiam Chye Lim).Chapter 25: Bringing Mesenchymal stem cells to clinic (RobertDeans).