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Autor: Jeremy W. Dale
ISBN-13: 9780470518700
Einband: E-Book
Seiten: 394
Sprache: Englisch
eBook Typ: PDF
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From Genes to Genomes

Concepts and Applications of DNA Technology
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"... an excellent book... achieves all of itsgoals with style, clarity and completeness... You can see thepower and possibilities of molecular genetics as youread..." -Human Genetics
"This volume hits an outstanding balance among readability,coverage, and detail." -Biochemistry and MolecularBiology Education

Rapid advances in a collection of techniques referred to as genetechnology, genetic engineering, recombinant DNA technology andgene cloning have pushed molecular biology to the forefront of thebiological sciences. This new edition of a concise, well-writtentextbook introduces key techniques and concepts involved in cloninggenes and in studying their expression and variation.

The book opens with a brief review of the basic concepts ofmolecular biology, before moving on to describe the key molecularmethods and how they fit together. This ranges from the cloning andstudy of individual genes to the sequencing of whole genomes, andthe analysis of genome-wide information. Finally, the book moves onto consider some of the applications of these techniques, inbiotechnology, medicine and agriculture, as well as in researchthat is causing the current explosion of knowledge across thebiological sciences.

From Genes to Genomes: Concepts and Applications of DNATechnology, Second Edition includes full two-colour designthroughout. Specific changes for the new edition include:
* Strengthening of gene to genome theme
* Updating and reinforcing of material on proteomics, genetherapy and stem cells
* More eukaryotic/mammalian examples and less focus onbacteria

This textbook is must-have for all undergraduates studyingintermediate molecular genetics within the biological andbiomedical sciences. It is also of interest for researchers and allthose needing to update their knowledge of this rapidly movingfield.

1 Introduction.

2 Basic molecular biology.

2.1 Nucleic acid structure.

2.2 What is a gene?

2.3 Information flow: gene expression.

2.4 Gene structure and organization.

3 How to clone a gene.

3.1 What is cloning?

3.2 Overview of the procedures.

3.3 Gene libraries.

3.4 Hybridization.

3.5 Polymerase chain reaction.

3.6 Extraction and purification of nucleic acids.

3.7 Detection and quantitation of nucleic acids.

3.8 Gel electrophoresis.

4 Cutting and joining DNA.

4.1 Restriction endonucleases.

4.2 Ligation.

4.3 Modification of restriction fragment ends.

4.4 Other ways of joining DNA molecules.

5 Vectors.

5.1 Plasmid vectors.

5.2 Vectors based on the lambda bacteriophage.

5.3 Cosmids.

5.4 M13 vectors.

5.5 Expression vectors.

5.6 Vectors for cloning and expression in eukaryotic cells.

5.7 Supervectors: YACs and BACs.

5.8 Summary.

6 Genomic and cDNA libraries.

6.1 Genomic libraries.

6.2 Growing and storing libraries.

6.3 cDNA libraries.

6.4 Random, arrayed and ordered libraries.

7 Finding the right clone.

7.1 Screening libraries with gene probes.

7.2 Screening expression libraries with antibodies.

7.3 Subcloning.

7.4 Characterization of plasmid clones.

8 Polymerase chain reaction.

8.1 The PCR reaction.

8.2 PCR in practice.

8.3 Cloning PCR products.

8.4 Long-range PCR.

8.5 Reverse-transcription PCR.

8.6 Rapid amplification of cDNA ends.

8.7 Quantitative PCR.

8.8 Applications of PCR.

9 Characterization of a cloned gene.

9.1 DNA sequencing.

9.2 Databank entries and annotation.

9.3 Sequence analysis.

9.4 Sequence comparisons.

9.5 Protein structure.

9.6 Confirming gene function.

10 Analysis of gene expression.

10.1 Analysing transcription.

10.2 Methods for studying the promoter.

10.3 Regulatory elements and DNA-binding proteins.

10.4 Translational analysis.

11 Products from native and manipulated cloned genes.

11.1 Factors affecting expression of cloned genes.

11.2 Expression of cloned genes in bacteria.

11.3 Expression in eukaryotic host cells.

11.4 Adding tags and signals.

11.5 In vitro mutagenesis.

11.6 Vaccines.

12 Genomic analysis.

12.1 Genome sequencing.

12.2 Analysis and annotation.

12.3 Comparing genomes.

12.4 Genome browsers.

12.5 Relating genes and functions: genetic and physicalmaps.

12.6 Transposon mutagenesis and other screening techniques.

12.7 Conclusion.

13 Analysis of genetic variation.

13.1 Single nucleotide polymorphisms.

13.2 Larger-scale variations.

13.3 Other methods for studying variation.

13.4 Human genetic diseases.

13.5 Molecular phylogeny.

14 Post-genomic analysis.

14.1 Analysing transcription; transcriptomes.

14.2 Array-based methods.

14.3 Translational analysis; proteomics.

14.4 Post-translational analysis: protein interactions.

14.5 Integrative studies; systems biology.

15 Modifying organisms; transgenics.

15.1 Modification of bacteria and viruses: live vaccines.

15.2 Transgenesis and cloning.

15.3 Animal transgenesis.

15.4 Applications of transgenic animals.

15.5 Transgenic plants and their applications.




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Autor: Jeremy W. Dale
ISBN-13 :: 9780470518700
ISBN: 0470518707
Verlag: John Wiley & Sons
Seiten: 394
Sprache: Englisch
Auflage 2, 2. Auflage
Sonstiges: Ebook