Mass Spectrometry for Microbial Proteomics
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Mass Spectrometry for Microbial Proteomics

 Buch
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ISBN-13:
9780470681992
Einband:
Buch
Erscheinungsdatum:
01.07.2010
Seiten:
534
Autor:
Haroun Shah
Gewicht:
1172 g
Format:
251x176x30 mm
Sprache:
Englisch
Beschreibung:

Preface
List of contributors Microbial Characterisation; the Transition from Conventional Methods to Proteomics.

1) CHANGING CONCEPTS IN THE CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY

Haroun Shah et al

2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES (THE CHANGE FROM TARGETED GENES TO PROTEINS)

Radhey Gupta

2: PROTEOMICS TOOLS AND BIOMARKER DISCOVERY.

3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT LINKS TO GENOMICS

Raju Misra.

4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN MICROORGANISMS

Ming Fang

Proteomic Strategies for Protein Identification

1. Bottom-up Proteomics

2. Top-down Proteomics

Multidimensional Protein Identification

Mass Spectrometry Based Targeted Protein Quantification and Biomarker Discovery

Selected Reaction Monitoring

Conclusions

5) MALDI MASS SPECTROMETRY IMAGING, A NEW FRONTIER IN BIOSTRUCTURAL TECHNIQUES: APPLICATIONS IN BIOMEDICINE Simona Francese and Malcolm R. Clench

3: PROTEIN SAMPLES PREPARATION TECHNIQUES

CONVENTIONAL APPROACHES FOR SAMPLE PREPARATION FOR LIQUID

CHROMATOGRAPHY AND TWO-DIMENSIONAL GEL ELECTROPHORESIS

Vesela Encheva and Robert Parker

7) ISOLATION AND PREPARATION OF SPORE PROTEINS AND SUBSEQUENT CHARACTERISATION BY ELECTROPHORESIS AND MASS SPECTROMETRY Nicola Thorne, Saheer Gharbia and Haroun Shah

8) CHARACTERIZATION OF BACTERIAL MEMBRANE PROTEINS USING A NOVEL COMBINATION OF A LIPID BASED PROTEIN IMMOBILIZATION TECHNIQUE WITH MASS SPECTROMETRY

Roger Karlsson, Darren Chooneea, Elisabet Carlsohn, Vesela Encheva and Haroun Shah

9) Wider Protein Detection from Biological Extracts by the Reduction of Dynamic Concentration Range.

Luc Guerrier, Egisto Boschetti and Piergiorgi Roghetti

10) 3D-gel electrophoresis - a new development in protein analysis.

Robert Ventzki and Josef Stegemann

SECTION 4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION.

11) Microbial Disease Biomarkers using ProteinChip Arrays

Shea Hamilton, Michael Levin, J. Simon Kroll, Paul R. Langford

12) MALDI-TOF MS and microbial identification: years of experimental

development to an established protocol.

Wibke Kallow, Marcel Erhard,

Haroun N. Shah, Emmanuel Raptakis, Martin Welker.

5: Targeted Molecules and Analysis of Specific Microorganisms.

13) Whole Cell MALDI Mass Spectrometry for the Rapid Characterisation of

Bacteria; A Survey of Applications to Major Phyletic Lines in Microbial

Kingdom.

Ben van Baar

Bacillus spp.

Staphylococcus spp.

Streptococcus spp.

Mycobacterium spp.

Other Gram-positive bacteria

Escherichia coli

Gram-negative food- and waterborne pathogen proteobacteria, other than E. Coli

Typical sexually transmitted pathogens: Neisseria spp. and Haemophilus spp.

Gram-negative biothreat agent bacteria

Other Gram-negative bacteria

Pathogenic Cyanobacteria

Strategies for the identification of biomarkers in whole cell MALDI MS spectra

Protein database consideration

On-target treatment and analysis

Off-target' Analysis and correlation with proteomics studies

General consideration of biomarker identification strategies

Conclusions and outlook

14) The power of Gel-based proteomics to understand

physiology in Bacillus subtilis

Haike Antelmann and Michael Hecker

Introduction

Results

1 Proteomics of protein secretion mechanisms in Bacillus subtilis

2 Definition of proteomic signatures to study cell physiology

3 Proteomics as tool to visualize reversible and irreversible thiol- modifications

4 Proteomics as tool to define regulon structures and targets for non- coding RNAs

5 Acknowledgment

15) Mass Spectrometry in the study of Tularemia Pathogenesis.

Jiri Stulik, Juraj Lenco, Jiri Dresler, Jana Klimentova, Lenka Hernychova, Lucie Balonova and Alena Fucikova.

References

16) Bacterial Post-G
Lieferung vom Verlag mit leichten Qualitätsmängeln möglich
Die neuesten Fortschritte in der Proteomik, die zum großen Teil durch die Entwicklungen in der Massenspektronomie vorangetrieben werden, zeigen immer wieder die Komplexität und Vielfalt pathogener Mechanismen in Mikroben als Verursacher und Verstärker infektiöser Erkrankungen. Eine neue Ära in der medizinischen Mikrobiologie erfordert daher einen schnellen Wechsel der heutigen Verfahren hin zu hoch leistungsfähigen Analysesystemen für die Diagnose mikrobieller Pathogene. Dieses Buch deckt die ganze Bandbreite mikrobiologischer Anwendungen der Proteomik und Massenspektronomie ab. In sechs Kapiteln werden die Abläufe dargestellt, mit denen die meisten mikrobiologischen Labors das Thema angehen: Übergang, Werkzeuge, Vorbereitung, Profiling-by-Pattern, Zielproteine und Datenanalyse.

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