Professor Haroun N. Shah is Head of Molecular Identification Services Unit at the Centre for Infections, Health Protection Agency, London. The Centre for Infections provides infectious disease surveillance and microbial identification services, co-ordinating the investigation and cause of national and uncommon outbreaks of diseases. Haroun holds several chairs at various universities and spent 25 years in academic life at the University of London.

Preface
 
List of contributors Microbial Characterisation; the Transition from Conventional Methods to Proteomics.
 
1) CHANGING CONCEPTS IN THE CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY
 
Haroun Shah et al
 
2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES (THE CHANGE FROM TARGETED GENES TO PROTEINS)
 
Radhey Gupta
 
2: PROTEOMICS TOOLS AND BIOMARKER DISCOVERY.
 
3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT LINKS TO GENOMICS
 
Raju Misra.
 
4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN MICROORGANISMS
 
Ming Fang
 
Proteomic Strategies for Protein Identification
 
1. Bottom-up Proteomics
 
2. Top-down Proteomics
 
Multidimensional Protein Identification
 
Mass Spectrometry Based Targeted Protein Quantification and Biomarker Discovery
 
Selected Reaction Monitoring
 
Conclusions
 
5) MALDI MASS SPECTROMETRY IMAGING, A NEW FRONTIER IN BIOSTRUCTURAL TECHNIQUES: APPLICATIONS IN BIOMEDICINE Simona Francese and Malcolm R. Clench
 
3: PROTEIN SAMPLES PREPARATION TECHNIQUES
 
CONVENTIONAL APPROACHES FOR SAMPLE PREPARATION FOR LIQUID
 
CHROMATOGRAPHY AND TWO-DIMENSIONAL GEL ELECTROPHORESIS
 
Vesela Encheva and Robert Parker
 
7) ISOLATION AND PREPARATION OF SPORE PROTEINS AND SUBSEQUENT CHARACTERISATION BY ELECTROPHORESIS AND MASS SPECTROMETRY Nicola Thorne, Saheer Gharbia and Haroun Shah
 
8) CHARACTERIZATION OF BACTERIAL MEMBRANE PROTEINS USING A NOVEL COMBINATION OF A LIPID BASED PROTEIN IMMOBILIZATION TECHNIQUE WITH MASS SPECTROMETRY
 
Roger Karlsson, Darren Chooneea, Elisabet Carlsohn, Vesela Encheva and Haroun Shah
 
9) Wider Protein Detection from Biological Extracts by the Reduction of Dynamic Concentration Range.
 
Luc Guerrier, Egisto Boschetti and Piergiorgi Roghetti
 
10) 3D-gel electrophoresis - a new development in protein analysis.
 
Robert Ventzki and Josef Stegemann
 
SECTION 4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION.
 
11) Microbial Disease Biomarkers using ProteinChip Arrays
 
Shea Hamilton, Michael Levin, J. Simon Kroll, Paul R. Langford
 
12) MALDI-TOF MS and microbial identification: years of experimental
 
development to an established protocol.
 
Wibke Kallow, Marcel Erhard,
 
Haroun N. Shah, Emmanuel Raptakis, Martin Welker.
 
5: Targeted Molecules and Analysis of Specific Microorganisms.
 
13) Whole Cell MALDI Mass Spectrometry for the Rapid Characterisation of
 
Bacteria; A Survey of Applications to Major Phyletic Lines in Microbial
 
Kingdom.
 
Ben van Baar
 
Bacillus spp.
 
Staphylococcus spp.
 
Streptococcus spp.
 
Mycobacterium spp.
 
Other Gram-positive bacteria
 
Escherichia coli
 
Gram-negative food- and waterborne pathogen proteobacteria, other than E. Coli
 
Typical sexually transmitted pathogens: Neisseria spp. and Haemophilus spp.
 
Gram-negative biothreat agent bacteria
 
Other Gram-negative bacteria
 
Pathogenic Cyanobacteria
 
Strategies for the identification of biomarkers in whole cell MALDI MS spectra
 
Protein database consideration
 
On-target treatment and analysis
 
Off-target' Analysis and correlation with proteomics studies
 
General consideration of biomarker identification strategies
 
Conclusions and outlook
 
14) The power of Gel-based proteomics to understand
 
physiology in Bacillus subtilis
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Die neuesten Fortschritte in der Proteomik, die zum großen Teil durch die Entwicklungen in der Massenspektronomie vorangetrieben werden, zeigen immer wieder die Komplexität und Vielfalt pathogener Mechanismen in Mikroben als Verursacher und Verstärker infektiöser Erkrankungen. Eine neue Ära in der medizinischen Mikrobiologie erfordert daher einen schnellen Wechsel der heutigen Verfahren hin zu hoch leistungsfähigen Analysesystemen für die Diagnose mikrobieller Pathogene. Dieses Buch deckt die ganze Bandbreite mikrobiologischer Anwendungen der Proteomik und Massenspektronomie ab. In sechs Kapiteln werden die Abläufe dargestellt, mit denen die meisten mikrobiologischen Labors das Thema angehen: Übergang, Werkzeuge, Vorbereitung, Profiling-by-Pattern, Zielproteine und Datenanalyse.