Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: wiley.com/go/murphy/lightmicroscopy

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website:wiley.com/go/murphy/lightmicroscopy

Chapter 1: Fundamentals of Light MicroscopyChapter 2: Light and ColorChapter 3: Illuminators, Filters, and the Isolation of Specific WavelengthsChapter 4: Lenses and Geometrical OpticsChapter 5: Diffraction and Interference in Image FormationChapter 6: Diffraction and Spatial ResolutionChapter 7: Phase Contrast Microscopy and Darkfield MicroscopyChapter 8: Properties of Polarized LightChapter 9: Polarization MicroscopyChapter 10: Differential Interference Contrast (DIC) Microscopy and Modulation Contrast MicroscopyChapter 11: Fluorescence MicroscopyChapter 12: Fluorescence Imaging of Dynamic Molecular ProcessesChapter 13: Confocal Laser Scanning MicroscopyChapter 14: Two-Photon Excitation Fluorescence MicroscopyChapter 15: Superresolution ImagingChapter 16: Imaging Living Cells With The MicroscopeChapter 17: Fundamentals of Digital ImagingChapter 18: Digital Imaging ProcessingAppendix IAppendix IIAppendix IIIGlossaryReferencesIndex