The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately.Flow cytometry is key in this diagnostic pathway.This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams.The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry.Practical Flow Cytometry in Haematology Diagnosis provides:* A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images* Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfallsAll those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

Preface, viiAcknowledgements, viii1 Introduction, 1References, 22 Principles of Flow Cytometry, 3Introduction, 3Sample preparation, 3The flow cytometer, 4The fluidic system, 4The optical system, 5The electronic system, 9Data display, 9Acknowledgements, 18References, 193 Limitations, 20Introduction, 20Clinical context issues, 20Sampling issues, 20Technical issues, 25Interpretation issues, 28Conclusion, 29References, 294 Normal Blood and Bone Marrow Populations, 31Normal stem and precursor cell populations, 31References, 415 Acute Leukaemia, 43Introduction, 43Identification of neoplastic precursor cells, 45Lineage assignment of acute leukaemia, 52Acute myeloid leukaemia, 54Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma), 77Examination of cerebro-spinal fluid and serous effusions, 88Identification of genetic abnormalities by flow, 92Conclusion, 93References, 936 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma, 100Normal peripheral blood lymphoid populations, 100Identification of clonal lymphoid populations, 101Identification of clonal B-cell disorders, 101CD5 positive B-cell lymphoproliferative disorders, 102CD10 positive B-cell lymphoproliferative disorders, 116B-cell disorders identified using an extended B-cell panel, 121Identification of clonal T-cell disorders, 125CD4 positive T-cell disorders, 128CD8 positive T-cell disorders, 136CD4/CD8 positive T-cell disorders, 141CD4/8 negative disorders, 141Flow cytometric assessment of serous effusions, 146Flow cytometric assessment of cerebrospinal fluid, 146Summary, 149References, 1497 Myelodysplastic Syndromes and Myeloproliferative Neoplasms, 152Introduction, 152Myelodysplastic syndromes, 152Myelodysplastic/myeloproliferative neoplasms, 163Chronic myeloid leukaemia, 165The BCR-ABL negative myeloproliferative neoplasms, 166Mastocytosis, 167Summary, 170References, 1708 Disorders of Plasma Cells, 173Plasma cell disorders, 173Current diagnostic criteria, 173Plasma cell morphology, 175The applications of immunophenotyping to plasma cell disorders, 178References, 1839 Minimal Residual Disease, 184Introduction, 184Minimal residual disease analysis in the management of acute lymphoblastic leukaemia, 187Conclusions, 199Acknowledgements, 200References, 20010 Red Cells, Leucocytes and Platelets, 202Paroxysmal nocturnal haemoglobinuria, 202Red cell membrane disorders, 208Foetal maternal haemorrhage, 211Lymphocyte subset analysisand immunodeficiency, 214Haemopoietic stem cell enumeration, 214Granulocyte disorders, 215Platelet disorders, 219References, 22111 Reactive and Non-neoplastic Phenomena, 225Peripheral blood, 225Bone marrow, 231Pleural and pericardial fluids, 236Bronchoalveolar lavage specimens, 238Cerebrospinal fluid, 238References, 240Index, 243