Discover how to use HILIC to analyze and better understand polar compounds An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications. Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring: Detailed examples of HILIC methods and development approaches Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods References to the primary literature at the end of each chapter Hydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.

Discover how to use HILIC to analyze and better understand polar compoundsAn increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book enables readers to develop a fundamental understanding of how HILIC works and then apply that knowledge to develop and implement a variety of practical applications.Hydrophilic Interaction Chromatography begins with discussions of HILIC retention mechanisms, stationary phases, and general method development. This sets the foundation for the book's extensive coverage of applications. The authors address unique separation challenges for bioanalytical, environmental, pharmaceutical, and biochemical applications. Moreover, there is a thorough discussion of HILIC in two-dimensional chromatography.With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC, Hydrophilic Interaction Chromatography serves as a practical guide for researchers, featuring:* Detailed examples of HILIC methods and development approaches* Thorough explanations of retention mechanisms and the impact of stationary phase and mobile phase properties on separations* Step-by-step guidance for developing efficient, sensitive, and robust HILIC methods* References to the primary literature at the end of each chapterHydrophilic Interaction Chromatography is written for scientists who use or develop analytical methods for the separation of polar compounds. In particular, these researchers will discover how HILIC can be used to analyze and better understand the composition of pharmaceutical, bioanalytical, biochemical, chemical, food, and environmental samples.

PREFACE xiiiCONTRIBUTORS xvCHAPTER 1 SEPARATION MECHANISMS IN HYDROPHILIC INTERACTION CHROMATOGRAPHY 1David V. McCalley1.1 Introduction 11.2 Historical Background: Recognition of the Contribution of Partition, Ion Exchange, and RP Interactions to the Retention Process 31.3 Recent Studies on the Contributory Mechanisms to HILIC Retention 71.4 Conclusions 38References 38CHAPTER 2 STATIONARY PHASES FOR HILIC 43Mohammed E.A. Ibrahim and Charles A. Lucy2.1 Introduction 432.2 HILIC Stationary Phases 442.3 Commercial HILIC Phases 612.4 Conclusions 77Acknowledgments 77References 77CHAPTER 3 HILIC METHOD DEVELOPMENT 87Yong Guo and Xiande Wang3.1 Introduction 873.2 General Method Development Considerations 883.3 HILIC Method Development 933.4 Detection for HILIC Methods 1043.5 Conclusions 107References 108CHAPTER 4 PHARMACEUTICAL APPLICATIONS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY 111Bernard A. Olsen, Donald S. Risley, V. Scott Sharp, Brian W. Pack, and Michelle L. Lytle4.1 Introduction 1124.2 Determination of Counterions 1174.3 Main Component Methods 1294.4 Determination of Impurities 1354.5 Excipients 1464.6 Chiral Applications 1524.7 Conclusions 161References 161CHAPTER 5 HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) FOR DRUG DISCOVERY 169Alfonso Espada and Mark Strege5.1 Drug Discovery Model 1695.2 HILIC Applications for In Vitro Biology 1705.4 Practical Considerations 1865.5 Conclusions 187References 188CHAPTER 6 ADVANCES IN HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) FOR BIOCHEMICAL APPLICATIONS 195Fred Rabel and Bernard A. Olsen6.1 Introduction 1956.2 Carbohydrates 1966.2.1 Mono- and Disaccharides 1966.3 Nucleobases and Nucleosides 2036.4 Oligonucleotides 2056.5 Amino Acids and Peptides 2066.6 Proteins 2096.7 Phospholipids 2096.8 Conclusions 211References 212CHAPTER 7 HILIC-MS FOR TARGETED METABOLOMICS AND SMALL MOLECULE BIOANALYSIS 219Hien P. Nguyen, Heather D. Tippens, and Kevin A. Schug7.1 Introduction 2197.2 The Role of HILIC-MS in Targeted Metabolomics versus Other LC Modes 2217.3 Strategies for Method Development Based on Retention Behavior of Targeted Metabolites on HILIC Stationary Phases 2237.4 Summary 231Acknowledgments 232References 232CHAPTER 8 HILIC FOR FOOD, ENVIRONMENTAL, AND OTHER APPLICATIONS 239Michael A. Koupparis, Nikolaos C. Megoulas, and Aikaterini M. Gremilogianni8.1 Introduction 2398.2 Food Applications for HILIC 2408.3 Environmental and Other Applications of HILIC 2548.4 Conclusions 257References 259CHAPTER 9 THEORY AND PRACTICE OF TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY SEPARATIONS INVOLVING THE HILIC MODE OF SEPARATION 265Stephen R. Groskreutz and Dwight R. Stoll9.1 Fundamentals of Multidimensional Liquid Chromatography 2659.2 Complementarity of HILIC Selectivity to Other Separation Modes 2789.3 Instrumentation and Experimental Considerations 2789.4 Applications 2919.5 The Future of HILIC Separations in 2DLC 298References 298INDEX 307