The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May Gr nwald Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis.This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors' institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis.The reader will review May.Grünwald.Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference.Practical Flow Cytometry in Haematology: 100 Worked Examples:* Provides a practical, example-based resource for flow cytometry* Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care* Includes both malignant and benign conditions* Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207)Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Preface, viiAcknowledgement, ixList of Abbreviations, xiTechnical Notes, xvLaboratory Values, xixCase 1 1Case 2 6Case 3 11Case 4 15Case 5 18Case 6 21Case 7 24Case 8 27Case 9 31Case 10 35Case 11 39Case 12 43Case 13 46Case 14 50Case 15 54Case 16 59Case 17 62Case 18 65Case 19 68Case 20 70Case 21 74Case 22 77Case 23 80Case 24 82Case 25 87Case 26 90Case 27 93Case 28 95Case 29 100Case 30 104Case 31 106Case 32 110Case 33 114Case 34 117Case 35 122Case 36 126Case 37 129Case 38 132Case 39 136Case 40 140Case 41 143Case 42 146Case 43 151Case 44 154Case 45 159Case 46 163Case 47 166Case 48 168Case 49 172Case 50 177Case 51 180Case 52 183Case 53 186Case 54 189Case 55 193Case 56 196Case 57 201Case 58 206Case 59 210Case 60 213Case 61 216Case 62 218Case 63 224Case 64 227Case 65 232Case 66 236Case 67 240Case 68 244Case 69 249Case 70 253Case 71 256Case 72 260Case 73 266Case 74 269Case 75 274Case 76 276Case 77 281Case 78 284Case 79 289Case 80 292Case 81 297Case 82 300Case 83 306Case 84 310Case 85 315Case 86 319Case 87 321Case 88 325Case 89 327Case 90 330Case 91 334Case 92 338Case 93 342Case 94 347Case 95 351Case 96 355Case 97 359Case 98 365Case 99 370Case 100 375Antibodies Used in Immunohistochemistry Studies, 381Flow Cytometry Antibodies, 386Molecular Terminology, 389Classification of Cases According to Diagnosis, 390Index, 391