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Autor: Shondra M. Pruett-Miller
ISBN-13: 9781493918614
Einband: Book
Seiten: 333
Gewicht: 783 g
Format: 262x187x20 mm
Sprache: Englisch

Chromosomal Mutagenesis

1239, Methods in Molecular Biology
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Includes all new cutting-edge methods and protocols for chromosomal mutagenesis research
1. Genome Editing by Targeted Chromosomal Mutagenesis

Dana Carroll

2. piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells

Stephen J. Pettitt, E-Pien Tan, and Kosuke Yusa

3. Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy

Jonathan M. Geisinger and Michele P. Calos

4. Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA

Faisal Reza and Peter M. Glazer

5. Genome Engineering Using Adeno-Associated Virus (AAV)

Rob Howes and Christine Schofield

6. Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization

Ryo Takeuchi, Michael Choi, and Barry L. Stoddard

7. Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform

Tomas Cermak, Colby G. Starker, and Daniel F. Voytas

8. Ligation-Independent Cloning (LIC) Assembly of TALENs

Jonathan L. Schmid-Burgk, Tobias Schmidt, and Veit Hornung

9. Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications

Sandrine Boissel and Andrew M. Scharenberg

10. Genome Engineering Using CRISPR-Cas9 System

Le Cong and Feng Zhang

11. Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases

Shondra M. Pruett-Miller and Gregory D. Davis

12. Endogenous Gene Tagging with Fluorescent Proteins

John Fetter, Andrey Samsonov, Nathan Zenser, Fan Zhang, Hongyi Zhang, and Dmitry Malkov

13. Silencing Long Non-Coding RNAs with Genome-Editing Tools

Tony Gutschner

14. Gene Editing Using ssODNs with Engineered Endonucleases

Fuqiang Chen, Shondra M. Pruett-Miller, and Gregory D. Davis

15. Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases

Kunitoshi Chiba and Dirk Hockemeyer

16. Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells

Matthew Porteus

17. Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models

Victoria M. Bedell and Stephen C. Ekker

18. Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection

Takehito Kaneko and Tomoji Mashimo

19. Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains

Takehito Kaneko
This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.

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Autor: Shondra M. Pruett-Miller
ISBN-13 :: 9781493918614
ISBN: 1493918613
Erscheinungsjahr: 01.01.2015
Verlag: Springer-Verlag GmbH
Gewicht: 783g
Seiten: 333
Sprache: Englisch
Auflage 15002, 2nd edition
Sonstiges: Buch, 262x187x20 mm, 39 schwarz-weiße und 22 farbige Abbildungen, 12 schwarz-weiße Tabellen