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Autor: Mónica V. Cunha
ISBN-13: 9781493920037
Einband: Book
Seiten: 536
Gewicht: 1123 g
Format: 265x189x38 mm
Sprache: Englisch

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

1247, Methods in Molecular Biology
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Includes cutting-edge methods and protocols
PART I- Introducing Nucleic Acid Testing In The Veterinary Laboratory

1. Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory

Mónica V. Cunha And João Inácio

2. Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice

Alicia Aranaz

3. Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory

Joseph Kozlovac And Beverly Schmitt

4. Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes

Tina Lombardo, Silvia Dotti, Riccardo Villa, Stefano Cinotti, And Maura Ferrari

5. Biological Specimen Collection And Processing for Molecular Analysis

Susan Sanchez And Holly Sellers

6. Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory

Nick Saunders and Ian Sharp

PART II - Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings

7. Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals

Fredrik Granberg, Oskar Karlsson, Mikael Leijon, Lihong Liu, and Sándor Belák

8. Real Time Reverse Transcriptase PCR For The Detection Of Bluetongue Virus

Carrie Batten, Lorraine Frost, And Chris Oura

9. Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues

Pedro Costa, Isabel Couto, Miguel Viveiros, and João Inácio

10. A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants

Florian Roeber, Aaron R. Jex, And Robin B. Gasser

11. Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches

Irene R. Grant And Linda D. Stewart

12. Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique

Hatem Soliman, Mona Saleh, And Mansour El-Matbouli

13. Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches

Jacinto Gomes And João Inácio

14. Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection

Ana Hurtado

15. DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp.

Christiane Schnee and Konrad Sachse

16. In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues

Maria Ballester and Fernando Rodriguez

17. Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections

Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Pors, Thomas Bjarnsholt, and Mette Boye

18. Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry

Joachim Frey and Peter Kuhnert

19. Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases

Mona Saleh, Hatem Soliman, and Mansour El-Matbouli

20. Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of Animal Pathogens

Fernando Teles And Luís Pina Fonseca

PART III - Molecular Profiling Of Veterinary Relevant Microbial Pathogens

21. Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms

Konrad Sachse and Petra Moebius

22. Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis

Ana Cláudia Carreira and Mónica V. Cunha

23. Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci

Ilda Santos-Sanches, Lélia Chambel, and Rogério Tenreiro

24. Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species

This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.

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Autor: Mónica V. Cunha
ISBN-13 :: 9781493920037
ISBN: 1493920030
Erscheinungsjahr: 01.01.2015
Verlag: Springer-Verlag GmbH
Gewicht: 1123g
Seiten: 536
Sprache: Englisch
Sonstiges: Buch, 265x189x38 mm, 30 schwarz-weiße und 26 farbige Abbildungen, 44 schwarz-weiße Tabellen, Bibliographie