Neuroproteomics

Neuroproteomics

Methods and Protocols
Jr. Stevens
Springer New York
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1027 g
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260x183x30 mm
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Includes fully updated cutting-edge techniques for the study of neuroproteomics

Neuroproteomics Studies: Challenges and Updates.- Progress and Potential of Imaging Mass Spectrometry Applied to Biomarker Discovery.- Biofluid Proteomics and Biomarkers in Traumatic Brain Injury.- Degradomics in Neurotrauma: Profiling Traumatic Brain Injury.- Evolving Relevance of Neuroproteomics in Alzheimer's Disease.- Genome to Phenome: A Systems Biology Approach to PTSD Using an Animal Model.- Photoaffinity Labeling of Pentameric Ligand-Gated Ion Channels to Identify: A Proteomic Approach to Identify Allosteric Ligand Binding Sites.- Quantitative Phosphoproteomic Analysis of Brain Tissues.- Glycoproteins Enrichment and LC-MS/MS Glycoproteomics in Central Nervous System Applications.- A Novel 2-DE-Based Proteomic Analysis to Identify Multiple Substrates for Specific Protease in Neuronal Cells.- Neuroproteomic Profiling of Cerebrospinal Fluid (CSF) by Multiplexed Affinity Arrays.- Isolation and Proteomic Analysis of Microvesicles and Exosomes from HT22 Cells and Primary Neurons.- Combined MALDI Mass Spectrometry Imaging and Parafilm-Assisted Microdissection-Based LC-MS/MS Workflows in the Study of the Brain.- De Novo and Uninterrupted SILAC Labeling of Primary Microglia.- Spike-In SILAC Approach for Proteomic Analysis of Ex Vivo Microglia.- A Proteomic Evaluation of Sympathetic Activity Biomarkers of the Hypothalamus-Pituitary-Adrenal Axis by Western Blotting Technique Following Experimental Traumatic Brain Injury.- Efficient and Accurate Algorithm for Cleaved Fragments Prediction (CFPA) in Protein Sequences Dataset Based on Consensus and Its Variants:  A Novel Degradomics Prediction Application.- Effect of Second-Hand Tobacco Smoke on the Nitration of Brain Proteins: A Systems Biology & Bioinformatics Approach.- An Advanced Omic Approach to Identify Co-Regulated Clusters and Transcription Regulation Network with AGCT and SHOE Methods.- AutoDock and AutoDockTools for Protein-Ligand Docking: Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1(BACE1) as a Case Study.- An Integration of Decision Tree and Visual Analysis to Analyze Intracranial Pressure.

This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central nervous system and CNS-related disorders. The detailed contents of this book include the exploration of several exciting areas of advanced methods used for neuroproteomics research including relative and absolute protein quantitation by mass spectrometry, characterization of post-translational modifications, as well as bioinformatics and computational approaches. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 
Updated and accessible, Neuroproteomics: Methods and Protocols, Second Editionserves researchers and clinical scientists involved in the area of biomarker research and protein biochemistry, as well as molecular biologists and biochemists who have been involved in proteomics research already or even for those new to the field.

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