Immobilisation of Non-activated Unfixed Platelets for Real Time Single-cell Analysis.- Imaging Platelets and Megakaryocytes by High Resolution Laser Fluorescence Microscopy.- Single Molecule Localisation and Structured Illumination Microscopy of Platelet Proteins.- Electron Tomography and Correlative Approaches in Platelet Studies.- Screening and High-throughput Platelets Assays.- High-throughput Signaling Profiling in Blood Platelets by Multiplexed Phosphoflow Cytometry.- Precise Quantification of Platelet Proteins and their Phosphorylation States.- The Study of Platelet Receptors using Artificial Lipid Bilayers.- Three Dimensional Culture in a Methylcellulose-based Hydrogel to Study the Impact of Stiffness on Megakaryocyte Differentiation.- Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor Driven Forward Programming.- Three-dimensional Tissue Models for Studying Ex Vivo Megakaryocytopoiesis and Platelet Production.- Fluorescence Approaches to Image and Quantify the Demarcation Membrane System in Living Megakaryocytes.- High-resolution 3D Imaging of Megakaryocytes using Focused Ion Beam-Scanning Electron Microscopy.- Optical Clearing of Murine Bones to Study Megakaryocytes in Intact Bone Marrow Using Light-sheet Fluorescence Microscopy.- Mathematical Techniques for Understanding Platelet Regulation and the Development of New Pharmacological Approaches.
The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or avoiding known pitfalls.
Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.
