Der Artikel ist weiterhin als ^^OTHERCONDITION^^ verfügbar.
Autor: Paul A. Clemons
ISBN-13: 9781627039079
Einband: Previously published in hardcover
Seiten: 228
Gewicht: 484 g
Format: 260x193x12 mm
Sprache: Englisch

Cell-Based Assays for High-Throughput Screening

Methods and Protocols
Geben Sie Ihre Bewertung ab!  
Wir verlosen jeden Monat unter allen freigegebenen Rezensionen
3 Gutscheine im Wert von 20 Euro. Teilnahmebedingungen
As the use of high-throughput screening expands, the need for detailed reference materials becomes ever more pressing. This book fills an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening.
Provides an easily accessible reference volume for cell-based phenotypic screening written by leading researchers in the field
Chapter 1: Introduction: Cell-based Assays for High-throughput Screening W. Frank An and Nicola J. Tolliday Chapter 2: High-throughput Screening of Model Bacteria Soumaya Zlitni, Jan E. Blanchard, and Eric D. Brown Chapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas Mayer Chapter 4: Large-scale Small-molecule Screen Using Zebrafish Embryos Charles C. Hong Chapter 5: Whole-animal High-throughput Screens: The C. elegans Model Eyleen J. O'Rourke, Annie L. Conery, and Terence I. Moy Chapter 6: Whole-organism Screening: Plants April Agee and David Carter Chapter 7: Fluorescence-based Assays W. Frank An Chapter 8: Reporter-gene Assays Andy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. Wong Chapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay Aruna D. Balgi and Michel Roberge Chapter 10: Assay for Isolation of Inhibitors of Her2-kinase Expression Gabriela Chiosis and Adam B. Keeton Chapter 11: ImageJ: A Primer Katherine Luby-Phelps Chapter 12: High-content Screening: Flow Cytometry Analysis Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. Sklar Chapter 13: High-throughput Real-time PCR for Detection of Gene-expression Levels Bridget K. Wagner and Zoltan Arany Chapter 14: Interpretation of Uniform-well Readouts Serene Josiah Chapter 15: Extracting Rich Information from Images Anne E. Carpenter
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Zu diesem Artikel ist noch keine Rezension vorhanden.
Helfen sie anderen Besuchern und verfassen Sie selbst eine Rezension.

 

Rezensionen

Autor: Paul A. Clemons
ISBN-13 :: 9781627039079
ISBN: 1627039074
Verlag: Humana Press
Gewicht: 484g
Seiten: 228
Sprache: Englisch
Auflage 2009.
Sonstiges: Taschenbuch, 260x193x12 mm