Protein Purification: An Overview.- Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE.- High Throughput Process Development: I. Process Chromatography.- High Throughput Process Development: II. Membrane Chromatography.- Media Selection in Ion Exchange Chromatography in a Single Microplate.- High Throughput Screening of Dye-Ligands for Chromatography.- Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips.- Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline.- Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants.- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins.- Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP).- Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm.- Immunoglobulin Purification by Caprylic Acid.- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview.- Expression and Purification of Large Active GST Fusion Enzymes.- Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins.- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery.- An Orthogonal Fusion Tag for Efficient Protein Purification.- Phage Display of Engineered Binding Proteins.- Biomimetic Affinity Ligands for Protein Purification.- Synthesis and Application of Dye-Ligand Affinity Adsorbents.- Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification.- Affinity Chromatography of Proteins on Monolithic Columns.- Sample Displacement Batch Chromatography of Proteins.- Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches.- Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications.- Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry.- Modern Bioanalysis of Proteins by Electrophoretic Techniques.- Protein Structure Validation and Analysis with X-Ray Crystallography.- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes.- Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview.- Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.- G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification.- Affinity Chromatography for Antibody Purification.- Screening and Purification of Recombinant Lignocellulolytic Enzymes.- Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFv.- One-Step Purification ofGlutamate decarboxylase from E. coli Using Aqueous Two Phase System.
Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.
